Degradation of regulatory proteins with short half-lives and of abnormal or misfolded proteins occurs in the cytosol, and requires ATP and ubiquitin. Named based on its presence in all eukaryotic cells, ubiquitin is a small (8.5 kDa, 76 residue) polypeptide that targets many intracellular proteins for degradation. The primary structure of ubiquitin is highly conserved. Only 3 of 76 residues differ between yeast and human ubiquitin. Figure 1 illustrates the three-dimensional structure of ubiquitin. Ubiquitin molecules are attached by non–α-peptide bonds formed between the carboxyl terminal of ubiquitin and the ε-amino groups of lysyl residues in the target protein (Figure 2). The residue present at its amino terminus affects whether a protein is ubiquitinated. Amino terminal Met or Ser residues retard, whereas Asp or Arg favor ubiquitination. Attachment of a single ubiquitin molecule to transmembrane proteins alters their subcellular localization and targets them for degradation. Soluble proteins undergo polyubiquitination, the ligase-catalyzed attachment of four or more additional ubiquitin molecules (Figure 1). Subsequent degradation of ubiquitin-tagged proteins takes place in the proteasome, a macromolecule that also is ubiquitous in eukaryotic cells. The proteasome consists of a macromolecular, cylindrical complex of proteins, whose stacked rings form a central pore that harbors the active sites of proteolytic enzymes. For degradation, a protein thus must first enter the central pore. Entry into the core is regulated by the two outer rings that recognize polyubiquitinated proteins (Figures 3 and 4).

Fig1. Three-dimensional structure of ubiquitin. Shown are α-helices (blue), β-strands (green), and the R-groups of lysyl residues (orange). Lys48 & Lys63 are sites for attachment of additional ubiquitin molecules during polyubiquitination. (Rogerdodd/Wikipedia)

Fig2. Reactions involved in the attachment of ubiquitin (Ub) to proteins. Three enzymes are involved. E1 is an activating enzyme, E2 a transferase, and E3 a ligase. While depicted as single entities, there are several types of E1, and over 500 types of E2. The terminal COOH of ubiquitin first forms a thioester. The coupled hydrolysis of PPi by pyrophosphatase ensures that the reaction will proceed readily. A thioester exchange reaction now transfers activated ubiquitin to E2. E3 then catalyzes the transfer of ubiquitin to the ε-amino group of a lysyl residue of the target protein. Additional rounds of ubiquitination result in subsequent polyubiquitination.

Fig3. Representation of the structure of a protea some. The upper ring is gated to permit only polyubiquitinated proteins to enter the proteosome, where immobilized internal proteases degrade them to peptides.

Fig4. An end-on view of a proteasome. (Thomas Splettstoesser/Wikipedia)

For the discovery of ubiquitin-mediated protein degradation, Aaron Ciechanover and Avram Hershko of Israel and Irwin Rose of the United States were awarded the 2004 Nobel Prize in Chemistry. Genetic disorders that result from defects in the genes that encode ubiquitin, ubiquitin ligases, or deubiquitinating enzymes include Angelman syndrome, autosomal recessive juvenile Parkinson disease, von Hippel-Lindau syndrome, and congenital polycythemia. For additional aspects of protein degradation and of ubiquitination, including its role in the cell cycle, see Chapters 4 and 35.

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اخبار العتبة العباسية المقدسة

في موكب العتبتين المقدستين.. المجمع العلمي ينظم محفله القرآني الدولي الأول بالنجف الأشرف

معمل ثلج الكفيل يرفع طاقته الإنتاجية لنحو 3 آلاف قالب يوميّاً خلال زيارة الأربعين

المجمع العلمي ينشر محطاته القرآنية على مختلف الطرق الرئيسة المؤدية إلى كربلاء

مجمع أبي الفضل العباس (عليه السلام) يرفع إنتاج الصمون لأكثر من 25 ألف قطعة يوميًا

المزيد

الآخبار الصحية

قد يؤدي إلى فشل القلب.. تحذير من مسكن آلام يستعمله الملايين

هل الجري يساعد على فقدان الوزن؟.. إليك ما يقوله الخبراء

ماذا يحدث لجسمك عند شرب عصير البطيخ بانتظام؟

علماء يكتشفون أن نقص عنصر غذائي "شائع" قد يسبب الزهايمر

المزيد

الآخبار التكنلوجية

المعادن الحيوية قد تنضب خلال 10 سنوات.. دراسة صادمة ؟

"إنستغرام" يستنسخ خاصية شائعة من منافسه "سناب شات"

رواد فضاء يغادرون محطة الفضاء الدولية بعد مهمة استمرت 5 أشهر

"كريساليس".. سفينة فضائية ستسافر بالبشر إلى أعماق النجوم ؟!

المزيد

مواضيع ذات صلة

Biosynthesis of the Nutritionally Nonessential Amino Acids

Nutritionally Essential & Nutritionally Nonessential Amino Acids

Cholesterol Synthesis, Transport, & Excretion: Clinical Aspects

Cholesterol is Excreted from The Body in The Bile as Cholesterol or as Bile Acids

Cholesterol is Transported Between Tissue in Plasma Lipoproteins

The Cholesterol Balance in Tissues is Tightly Regulated

Cholesterol Synthesis is Controlled by Regulation of HMG-CoA Reductase

Cholesterol is Biosynthesized From Acetyl-CoA

Brown Adipose Tissue Promotes Thermogenesis

Hormones Regulate Fat Mobilization

Adipose Tissue is The Main Store of Triacylglycerol in The Body

Classification of Proteins